Expression of mechanical stress related genes in subchondral bone of rat osteoarthritis model

doi:10.3969/j.issn.2095-4344.2013.22.014

Abstract

Abstract:

BACKGROUND: Changes of mechanical stress is a key factor for the development of osteoarthritis.
OBJECTIVE: To observe the expression of mechanical stress related genes in subchondral bone of rat osteoarthritis model caused by joint instability, and to provide basis for elaborating the effect of mechanical stress factors in the pathogenesis of osteoarthritis.

METHODS: Sprague Dawley rats were divided into two groups: experimental group and control group, 30 rats in each group. The right knee joints of experimental group underwent both medial collateral ligament resection and medial meniscectomy, while the control group just cut joint capsule. Rats were sacrificed at 1, 2 and 4 weeks after surgery to obtain the right knee joints. Total RNA of the subchondral bone was extracted, and the global gene expression of subchondral bone was researched with DNA microarray technology. The expression of mechanical stress related genes in subchondral bone of rat osteoarthritis model was explored according to the existing literatures, and then the results were identified through quantitative real-time PCR.
RESULTS AND CONCLUSION: Three important mechanical stress related differentially expressed genes included: Postn, Ihh and Bmp5, and were confirmed by quantitative real-time PCR. In the experimental group, Postn was significantly expressed at 1 week after operation, and the difference between two groups was significant (P < 0.05), and 2 weeks after operation, Postn was still highly expressed, but there was no significant difference between two groups (P > 0.05). Bmp5 was significantly expressed at 1 week after operation, but the difference between two groups was significant (P < 0.05), and at 2 weeks after operation, Bmp5 was highly expressed, but there was no significant difference between two groups (P > 0.05). Ihh was highly expressed at 1 and 2 weeks after operation, and the difference between two groups was significant (P < 0.05). Mechanical stress is the key stress in osteoarthritis. Further research of mechanical stress related genes and their pathway might provide more evidence for pathogenesis of osteoarthritis, thus discovering the targets for early diagnosis and early treatment.

Key words: bone and joint implants, bone and joint biomechanics, osteoarthritis, subchondral bone, mechanical stress, Postn, Bmp5, Ihh, rat osteoarthritis model, joint instability, gene chip;, whole genome;, 863 program

CLC Number:

R318

Fang Hang, Zhang Rong-kai, Chen Yu-xian, Song Yan-cheng, Cai Dao-zhang. Expression of mechanical stress related genes in subchondral bone of rat osteoarthritis model[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(22): 4084-4090.

Figures/Tables 3

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